Acquiring adaptive cell growth is critical for the biotech industry. To promote the final production of pharmaceutical products, there is an industry-leading approach that now leads to higher cell densities and protein titers.
Using small bags used in cell culture, several manufacturers have identified unmatched cell growth.
Hammond & al-Amgen1 have discovered a volatile product, bis(2,4-di-tetra-butylphenyl) phosphate (bDtBPP), derived from an antioxidant commonly used in polyethylene films. However, these antioxidants are essential for film absorption. They protect the polymer from oxidation during film removal and gamma irradiation. Therefore, the proper optimization and management of these components are very important to ensure that the cells grow properly.
Sartorius has taken on the challenge of working cell in a portable bag with our Flexboy® bag appearing in our new Flexsafe® series.
The beautiful cell growth in our bag is the result of the greatest antioxidant package. Triphosphate (2,4-di-tetra-butylphenyl) does not come into contact with the Flexboy® container. For the new Flexsafe® bags, we carefully reduce and control the concentration. Cell growth performance is determined and demonstrated through cell growth testing and extraction studies.
The S80 resin film of the Flexsafe® wallet is optimized with a lens design to ensure constant cell growth.
Positive cell proliferation
The antioxidant compound used to make the PE S80 film of the Flexsafe® container is designed to limit the concentration of Tris phosphate (2,4-di-tetra-butylphenyl). It prevents the formation of the degraded product, bis(2,4-di-tetra-butylphenyl) phosphate (bDtBPP), which interferes with cell growth (1)
Reproductive cell growth
Resin synthesis, antioxidant control, and regenerative regulation are regulated to ensure reproductive cell growth. Cell growth is indicated by a cell growth test. It is now widely believed that no effective removal is possible.